Correlation between Serum MicroRNA-150 Level and Renal Function Damage and Lipid Index in Children with Primary Nephrotic Syndrome

  • Jianwei Ji
Keywords: Primary nephrotic syndrome; Children; Serum microRNA-150; Blood lipid index

Abstract

MicroRNAs exist in body fluids and may have potential as biomarkers of disease. This study explored the clinical value of serum levels of microRNA-150 and serum lipid as biomarkers of idiopathic primary nephrotic syndrome in children. We studied 21 patients with primary nephrotic syndrome (68.75% steroid-sensitive nephrotic syndrome and 31.25% steroid-resistant nephrotic syndrome) and 10 healthy controls. The number of cells and the location of WT1 were determined by immune fluorescence assay, and the levels of serum microRNA-150 and blood lipid were detected by RT-q PCR. In the serum of patients with nephrotic syndrome, the expression level of microRNA-150 was lower than that of healthy controls, but only the expression level of microRNA-150 showed a significant decrease (p = 0.019). The expression of microRNA-193a in NS patients only increased slightly. Blood lipid index also showed an upward trend. The low expression and delocalization of nephroblastoma proteins in patients with nephroblastoma contribute to the loss of renal tissue cells, while the regulation of nephroblastoma proteins is not related to the level of microRNA-150 in patients'serum.

Published
2019-09-01