Effects of Latanoprost on Expression of TGF-β1 and Wnt/β-Catenin Signaling Pathway in Choroid of Form Deprivation Myopia Rats

  • Meng Wang
Keywords: Latanoprost; Choroid; Form deprivation myopia; TGF-β1; Wnt/β-catenin signaling pathway

Abstract

In this study, we investigated the effect of latanoprost on the expression of TGF- β1 and Wnt / β-Catenin signal pathway in the choroid of form deprivation myopia model rats. Compared with the control group, there was a significant difference in the axial length of FDM model group (P< 0.05). There was significant difference in the expression of TGF- β1 protein and mRNA between the two groups (P<0.05). The cultured cells were identified as choroidal fibroblasts by immunocytochemistry. There was no significant difference (P>0.05) in the comparison of GSK3 β protein in choroidal fibroblasts of rats in each group. TGF-β 1 and APC protein in FDM group were significantly lower than those in the control group (P<0.05), while dcl3, p21-gsk3 β and β - Catenin protein were significantly higher (P<0.05), and there was no significant difference (P>0.05) in the ratio of various indexes protein in FDM + ddk1 group and in the comparison of TGF - β1 and APC protein in FDM + ddk1 group and FDM group The expression of dcl3, p21-gsk3 β and β- Catenin decreased significantly (P<0.05). There was no significant difference in the expression of GSK3 β mRNA in the choroidal fibroblasts of each group (P>0.05). The expression of TGF-β 1 and APC mRNA in FDM group was significantly lower than that in the control group (P<0.05), while the expression of dcl3, p21-gsk3 β and β-catenin mRNA in FDM + ddk1 group was significantly higher than that in the control group (P<0.05) >In FDM + ddk1 group, TGF-β 1 and APC mRNA were significantly lower than those in FDM group (P<0.05), while dcl3, p21-gsk3 β and β-Catenin mRNA were significantly higher (P<0.05).

Published
2020-03-01