Effect of Combined Leech Injection Postconditioning and Ischemic Postconditioning on the Expression of PI3K/AKt Signal Pathway against Ischemia-reperfusion Injury in Rats
To observe the effect of combined leech injection postconditioning and ischemic postconditioning(IPOC) on the expression of relative genes and proteins of PI3K/AKt signal pathway against ischemia-reperfusion injury in rats, including p-AKt, PI3K, p-GSK 3β, p-eNOS in rats. The MCAO and IPOC models were made by suture occlusion method in order to measure the expression of relative genes and proteins of PI3K/AKt signal pathway by using Real time PCR and Western blot separately. The infarct volume of brain tissue was significant smaller in IPOC group than that of MCAO group after 24h following I/R(P<0.05). Leech injection postconditioning could further reduce infarct volume than that of IPOC (P<0.05)). The rate of apoptosis neuron of rats in MCAO increased markedly after reperfusion than the SO group’s (P<0.01). IPOC could make apoptosis neuron’s rate decrease(P<0.01). Leech injection postconditioning could further decrease the rate of apoptosis neuron than that of IPOC (P<0.05). Both p-AKt, PI3K, p-eNOS, p-GSK3β mRNA and their proteins’ expressions increased at 24h following reperfusion in MCAO(P<0.01 or 0.05). IPOC could increase their expression (P<0.05 or 0.01) than that of MCAO. Leech injection postconditioning could further induce their expression than that of IPOC (P<0.05 or 0.01). our results show that IPOC could protect neurons through upregulating the expression of PI3K/AKt signal pathway. Leech injection postconditioning could augment the neuroprotection induced by IPOC.