Expression of Matrix Metalloproteinase-28 in Gliomas and Its Effect on Proliferation and Invasion

  • Xi Chen
Keywords: Matrix metalloproteinase-28; Glioma; Proliferation and invasion


To evaluate the expression of matrix metalloproteinase-28 and explore its molecular mechanism in glioma cells.
Micro RNA (MicroRNA) reverse polymerase chain reaction was used to analyze matrix metalloproteinase-28
expression and transforming growth factor (TGF) -β expression in glioma patients and healthy volunteers. MTT
assays were performed to determine cell growth and metastasis, and performed separately. Annexin iodine
staining is also used to measure apoptosis. Matrix metalloproteinase-28 TGF-β protein expression was measured
by immunoblot analysis. Compared with the normal group, the expressions of MMP-28 and TGF-β were downregulated
in glioma patients. The overall survival and disease-free survival of patients with low MMP-28
expression were lower than those of high MMP-28 expression. Matrix metalloproteinase-28 TGF-β induces
overexpression of protein expression, and the difference of matrix metalloproteinase-28 inhibits the protein
expression of TGF-β glioma cells. MMP-28 reduces the difference in cell growth and apoptosis of these genes
by inhibiting glioma cells. In contrast, MMP-28 induced cell growth and reduced apoptosis of glioma cells that
activated TGF-β. In addition, TGF-β inhibitors attenuate the effects of matrix metalloproteinase-28 on glioma
cells. Overall, the results show that matrix metalloproteinase-28 can induce TGF-β in human glioma cells.